Wound healing is a complex physiological process that involves blood clotting, inflammation, cell proliferation, and tissue remodeling. It is how the body replaces damaged tissue and repairs injuries. Nanoparticles have gained attention in biomedical applications because they are effective, affordable, and less toxic. In this study, green-synthesized titanium dioxide (TiO₂) and selenium (Se) nanoparticles were prepared to evaluate their wound healing potential and antibacterial activity when incorporated into chitosan/PVP films. Low and high doses of TiO₂, 1% and 5%, and 5% Se nanoparticles were added to chitosan/PVP films to create wound dressings. These films were tested against common wound bacteria including E. coli, S. aureus, K. pneumoniae, Enterococcus, and Pseudomonas. The high-dose TiO₂ films showed the strongest antibacterial effect, with inhibition zones ranging from 34 to 36 millimeters. The low dose TiO₂ films and Se films were effective as well, but their activity was lower and varied between different bacterial strains. For wound healing, circular wounds were created on mice and treated with the films. Wound areas were measured on days 0, 3, 5, 7, and 14. The chitosan/PVP/TiO₂ 5% film produced the fastest healing, with nearly complete closure by day 14 reaching 99.9%. The 1% TiO₂ and 5% Se films improved healing compared to untreated controls, but the wounds closed more slowly and showed less tissue regeneration. Measurements and photographs confirmed that higher TiO₂ concentration consistently reduced the wound area throughout the study. These results show that green-synthesized TiO₂ and Se films can support faster wound healing and prevent bacterial infections. The 5% TiO₂ film was the most effective and could be studied further as a safe and practical wound dressing.
